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After acid hydrolysis and extraction, the total radioactivity of the monoacylglycerols was determined. Both pathways operated effectively in peroxisomes. The oxidation of lignoceric acid and the oxidation of butyryl-coenzyme A was not inhibited by rotenone (2 microM) or by antibodies against 3-ketoacyl-CoA thiolase. The oxidation of C14-CoA and the oxidation of C15-CoA were both inhibited, but differently, by antibodies against the beta- and alpha-hydroxylases, respectively. The formation of C14- and C15-fatty aldehydes, and of keto- and omega-fatty acids were also measured after hydrolysis of monoenoic monoglycerides. All of these compounds were formed from C14 and from C15. However, only C14-fatty aldehydes were formed from C14-CoA and C15-fatty aldehydes from C15-CoA. 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